How To Hide Blood From Luminol

She also shows how the chemical Luminol can reveal hidden blood stains that the eye alone cannot see. David says:. Do what is necessary to keep yourself out of harm’s way. In this test, luminol powder (C 8 H 7 O 3 N 3) is mixed with hydrogen peroxide (H 2 O 2) and a hydroxide (e. Yeshion, T. - 내 마룻바닥이 젖잖아요 - 그건 루미놀이랍니다. Luminol is created by mixing two chemicals together, producing a chemical reaction that causes blood to illuminate under specific lights. Chlorine bleaches can remove a Bloodstain to the naked eye but fortunately, forensics experts can use the application of substances such as luminol or phenolphthalein to show that haemoglobin is present. Luminol (C 8 H 7 N 3 O 2) is a chemical that exhibits chemiluminescence, with a blue glow, when mixed with an appropriate oxidizing agent. For example, hydrogen peroxide reacts when it contacts blood, causing a foaming effect. Luminol is also used on police crime dramas (Dexter, etc. But she says in a real lab, experts would use pig blood to conduct their tests. Experienced investigators can make a reliable identification based on how quickly the reaction occurs. They are indoor cats and I groom them often enough to know that there are no fleas or ticks present. Luminol is only considered as a presumptive test for blood because there are other substances capable of giving a positive reaction with luminol itself; these other substances may be bleach, plant peroxides and some metals, the reaction between luminol and these other substances are called false positives [7]. Many cleaning products such as those used to clean showers will cause luminol to react. management Subject: Re: Mink: Night before Opening - Minks Mgmt It was a very intricate and expensive system but one that Danny had really pushed for when security was being designed. Results: Inter-individual variation in oxidant levels was significantly greater than intra-individual variation (plasma, p<0. With the amount of bleeding present, this was obviously the first crime scene. Use club soda, hydrogen peroxide, ammonia, or baking soda. The respiratory burst of bronchoalveolar lavage AM was studied in 12 subjects with asthma and in five control. In newly released evidence, a photo entitled "luminol photos" show luminol, which investigators used to find cleaned up blood splattered throughout the salon. 뭐 하는지 알겠군요 피를 찾고 있는 거요? My husband didn't do anything. Asthma severity was classified using NHLBI guidelines. Luminol's presence may prevent other tests from being performed on a piece of evidence. courtesy of Howstuffworks. It dries quickly. Subsequent applications of Luminol will be ineffective. The chemical luminol, a component of BLUESTAR®, is oxidized by hydrogen peroxide, to produce a molecule in an excited state. So first you will have to arrange these things to do it which are 2 500ml bottles, 1/2 LB table salt, measuring cup, kitchen scale (optional) and pan for heating. DNA-analysis of the blood traces is still possible after luminol treatment. Results of ABO blood grouping from dried blood- stains treated with luminol reagent by absorption elution technique (n = 35). Bloodstains will show up in a bright blue colour. Teams of investigators arrive at a crime scene armed with special fact-finding gear. You know what I mean, it's in those light sticks you can snap to get colored light for a couple of hours. Sulphur hexaflouride is much denser than air. "He is trying to find a place to hide her body … he is trying to find a means of disposing of her body. #1: It wasn’t blood. Targeted gene therapy has been hampered by the inability to correct mutations in stem cells that can reconstitute the immune system after transplant into patients. Many cleaning products such as those used to clean showers will cause luminol to react. Several chemicals in luminol react with the hemoglobin found on the crime scene, resulting in a light-producing chemical reaction. Luminol in Forensics Introduction In 1928 Chemist Albrecht discovered chemical that emitted blue glow In 1937, scientist Specht used chemical on bloody items In 1951 Grodsky used chemical with sodium carbonate to detect blood Improvement made in 1966 by Weber which replaced. gl/y4V7w3 Chem­istry isn't just in­ter­est­ing and im­pres­sive, it's also a use­ful sci­e. So far, no luck. Luminol reacts with faecal matter, causing the same glow as if it were blood. So, luminol glows more or less depending on the concentration of the blood in the solution. In the luminol reaction however, both hemoglobin and peroxidases are acting catalytically. An archery stabilizer reduces the amount of shock and recoil caused by the relea. The Blood Lamp arouses fear with just the mention of its name; the unique lamp demands the blood of the user as payment before it will cast its bluish glow on you. The Donald is a never-ending rally dedicated to the 45th President of the United States, Donald J. If luminol reveals apparent blood traces, investigators will photograph or videotape the crime scene to record the pattern. for diluting the blood by spraying too much Luminol. Luminol testing also reacts to coppery substances, plant enzymes and excrement, raising an alternate explanation for the luminol results in the hallway. A personal note: “I never visit chic stores. ESR scientist Dianne Crenfeldt told the High Court in Auckland on Thursday that, with the help of a chemical test called luminol, scientists could clearly identify two patches of blood on the. To detect invisible blood stains, the luminol test is used, which is a chemical sprayed on carpets and furniture which reveals a slight phosphorescent light in the dark where bloodstains (and certain other stains) are present. Lifting his face mask, a gloved finger made the transiever burst to life. Luminol (C 8 H 7 N 3 O 2) is a chemical that exhibits chemiluminescence, with a blue glow, when mixed with an appropriate oxidizing agent. This is the simplified reaction mechanism for the production of light by luminol. Scientists can take advantage of the luminol reaction to locate potential blood evidence that would be undetectable through visual examination. A good lawyer can contest circumstantial evidence and some physical evidence as well, including luminol findings. Use club soda, hydrogen peroxide, ammonia, or baking soda. Unit: Blood Analysis This unit begins with presumptive testing methods such as the use of luminol and the Kastle Meyer test. The oxygen produced from the hydrogen peroxide then reacts with the luminol dianion. If the small, characteristically shaped crystals form in the sample, it is blood. is a common chemiluminescent material. Typically, luminol only shows investigators that there might be blood in an area, since other substances, including household bleach, can also cause the luminol to glow. Small spots peeked out from under the edge of the bed where the old polyester blanket touched the carpeting. Interestingly, we have found that some of the spiked SeCN − decomposed to cyanide in human whole. which included luminol tests, later showed two circles of blood on the apartment floor. The production of light from luminol is a fairly complex redox reaction. "The blood that was on the road consisted of three pools – one larger pool and two smaller pools adjacent," he says. On each fabric, spots of blood was dotted, and the dried-bloodstained fabrics were hand washed with tap water, soaked in tap water with detergent and then hand washed, for 5 and 10 min. The luminol chemiluminescence reaction is responsible for the glow of lightsticks. If the crystals do not form then the test has failed to indicate blood. Luminol is created by mixing two chemicals together, producing a chemical reaction that causes blood to illuminate under specific lights. If only the defendant were here, she would have had some luminol (Luminol turns blue when in contact with blood) and I could check if this really is blood. ) to detect hidden blood spatters when spayed with Luminol and exposed to UV light. She sprays a spot on the ladder a few times as an example, and then passes the buck to you. I know this is true. To spray the Luminol, use to touch the areas you want to test. But no amount of luminol will help uncover bloodstains that have been painted over. Since I am a woman, I get blood on my shower floor every month. Transfused patients were more likely to have been taken back to the operating room. Detectives hoped to learn more using a chemical called luminol, which emits a distinctive glow when it comes in contact with blood. - 내 마룻바닥이 젖잖아요 - 그건 루미놀이랍니다. gl/y4V7w3 Chem­istry isn't just in­ter­est­ing and im­pres­sive, it's also a use­ful sci­e. Steve Krejci. Forensic tests such as luminal tests rely on the ability of blood to uptake oxygen: A protein in the blood called hemoglobin (responsible for transporting oxygen throughout the body) reacts with hydrogen peroxide and gives a positive test result. Ever wonder how detectives find people's fingerprints at a crime scene? It's actually not as hard as you may think. eu jpfules. Hide details Name: Date: Forensic Blood Lab WorksheetsPART 1 BLOOD TYPESPurpose: Identify specific blood types by visualizing agglutination. Typically, luminol only shows investigators that there might be blood in an area, since other substances, including household bleach, can also cause the luminol to glow. Luminol is also used on police crime dramas (Dexter, etc. Criminalistics Unit 3 Jeopardy Style Review Game. It is used at crime scenes and makes a major mess. ” “What is that… lum. ] Steven Avery had requested Teresa Halbach that day. Paint will "cover" blood, but,the blood is still there and painting over the blood actually seals the proteins in place. My understanding is Blue Star is better at recognizing blood that has been diluted or cleaned. Antiquity 66:24-35. Experienced investigators can make a reliable identification based on how quickly the reaction occurs. Chlorine bleaches can remove a Bloodstain to the naked eye but fortunately, forensics experts can use the application of substances such as luminol or phenolphthalein to show that haemoglobin is present. After cleaning the area with bleach, apply Luminol to the entire surface and walls. (B) Sketch of self-assembly of the CLP conjugate into a core-shell structured nanoparticle. CD177 had higher expression in naïve-RA than MTX-RA patients (MFI: 46620, 34475, p=0. In this test, luminol powder (C 8 H 7 O 3 N 3) is mixed with hydrogen peroxide (H 2 O 2) and a hydroxide (e. It is far less sensitive then luminol and phenolphthalein. Of all head and neck free flap patients, 48. Report comment. Enzymes in a variety of biological systems may also catalyse the decomposition of hydrogen peroxide. The limits of detection (LOD) and quantification (LOQ) were 73. Editorial Policies; Author Guidelines. Judge Nencini pointed out that it’s utterly inconceivable that the Luminol revealed anything other than blood, given the undisputed presence of a lot of blood at the cottage. Chlorine-based detergents contaminate blood, but leave behind intact hemoglobin. I know this is true. Luminol reacts with faecal matter, causing the same glow as if it were blood. 7 AM (MOCK WEEK INVESTIGATION) [The storm outside still rages on, several hours later. Just like in those crime scene investigation shows, you can reveal blood stains on floor tiles that have been cleaned up with luminol. Don't even think of writing a medical mystery without the wise counsel of Dr. blood stains are red, blood stains are red, i watch enough, murder shows, theyll never find you they ll never find you, funny punny poem, true crime lover, true crime convention great idea, true crime convention great, true crime convention great idea, ultraviolet, red ultraviolet lights, red ultraviolet lights, how to remove blood stains from. The glow lasts for about 30 seconds and is blue. Blood that sits around on the floor for a few minutes will form clots like big sheets of instant pudding. Talk with your doctor. Spray the area on the left of the ladder, just above the top rung. Yeshion, T. Peng Sijue repeatedly checked and noted, "The left side of the closet door and the left side of the ground have splattered blood. Luminol is created by mixing two chemicals together, producing a chemical reaction that causes blood to illuminate under specific lights. Sulphur hexaflouride is much denser than air. • Several chemicals in luminol react with the hemoglobin found on the crime scene, resulting in a light-producing chemical reaction. Thankfully their effort was fruitless, so they brought everything to Abby before taking a well-deserved break. courtesy of Howstuffworks. From the opening notes of first track ‘Place to Hide’, it becomes immediately obvious that this is something special. Yeshion 2004 The Use of Luminol as a Presumptive Blood Test for Prehistoric Archaeological Artifacts. Can my killer use a paralytic drug to disguise a murder using a gun as a suicide? Was it possible to uncover Laudanum in a corpse in 1941? Can toxicological testing distinguish Ativan from Restoril or Valium? Will spraying blood products over a bloody crime scene obscure Luminol's determination of the original blood pattern?. Luminol testing also reacts to coppery substances, plant enzymes and excrement, raising an alternate explanation for the luminol results in the hallway. Even on luminol assay, after PMA stimulation, there was higher ROS production in naïve-RA than MTX-RA. So far, no luck. Hide Caption ( - ) Photograph by. Blood that sits around on the floor for a few minutes will form clots like big sheets of instant pudding. too much luminol). 뭐 하는지 알겠군요 피를 찾고 있는 거요? My husband didn't do anything. Run, call for help, or hide. First of all, luminol does not react with blood alone. Within moments it would become apparent if there was evidence of murder. ) to detect hidden blood spatters when spayed with Luminol and exposed to UV light. Interview Recording Systems. The light, or luminescence, emitted in the luminol reaction is thought to result when an oxidizing agent, such as blood, catalyzes the oxidation of luminol by hydrogen peroxide in a basic solution. The Luminol is indeed reacting with the iron in the hemoglobin, and part of the mixture that makes Luminol is Hydrogen peroxide. The chemical luminol, a component of BLUESTAR®, is oxidized by hydrogen peroxide, to produce a molecule in an excited state. Substances other than blood (2–6) can catalyse the CL reaction that is the cen-tral component of the luminol test. 9% received blood transfusions. 11-01 1 sample of a Luminol-revealed blood trace was taken from Guede’s apartment. Results of ABO blood grouping from dried blood- stains treated with luminol reagent by absorption elution technique (n = 35). (B) Sketch of self-assembly of the CLP conjugate into a core-shell structured nanoparticle. If the small, characteristically shaped crystals form in the sample, it is blood. Steve Krejci. Luminol reacts with the hydroxide ion, forming a dianion. Luminol must be used very cautiously because once it touched blood and chemically reacted, at the same time it would also affect the follow-up testing of the blood contents. You see some smudges on the refrigerator shelf-thing. Peanut Butter Milkshake, it is definitely not flea dirt. Using Luminol® to test for the presence of blood in works of art. LUMINOL & BLUESTAR By: Ayesha Khawar, Catherine Lopez, Rebecca Murphy, and Sadie Azizi HISTORY • • • • • Luminol is a liquid solution that is sprayed to reveal traces of blood at crime scenes. Don't hide your eyes I know cause Luminol will make the blood glow And tho'tomorrow is bound to fall I told you we would make it but you ain't trying to hear it Now there's blood on the floor What you mad at me for I only called you cause I'm high I only called you cause I'm high I did not wanna see you cry or could I dry your eyes. making, that MP was trying to hide his involvement at first, before he finally said the hell with it and called 911. What I say out loud: “Did you know that blood doesn’t naturally fluoresce? It will not show up under a blacklight like in the movies. Today we tell about this special list. Snake venom meets blood. But she says in a real lab, experts would use pig blood to conduct their tests. Neither Sian Brooke (“Sherlock’s” Faith) nor Gina Bramhill (“Culverton Smith’s” Faith) actually has one, nor the therapist at the end of the episo. 44 shows two attempts at cleaning the soles of a shoe. Hide Caption ( - ) Photograph by. The analyst would then wait and check the sample under a microscope. But it is safer for the scientists to use. Forensic tests such as luminal tests rely on the ability of blood to uptake oxygen: A protein in the blood called hemoglobin (responsible for transporting oxygen throughout the body) reacts with hydrogen peroxide and gives a positive test result. How To : Find Hidden Blood Splatter Stains on Your Walls with This Infrared Photography Hack I'm sorry to say, but Dexter Morgan hasn't thought of everything. “If you want to ascertain the presence of blood on a knife, or even on clothes, the best method would be a luminol test1. If you don't do the Luminol, I would definitely try something like Noopept, but it is strong, and you may need something like the Picamilon to balance that out, or Phenibut HCL, which is just GABA, but with the phenyl attachment so that it crosses the blood brain barrier right away, to add a natural calm that works right away. Taken with Canon 50mm lens. Designed to provide all essential materials required for the chemiluminescent Luminol method. Thus, Bo Jinyan chose only one area to use the luminol. The Blood Lamp arouses fear with just the mention of its name; the unique lamp demands the blood of the user as payment before it will cast its bluish glow on you. Targeted gene therapy has been hampered by the inability to correct mutations in stem cells that can reconstitute the immune system after transplant into patients. On each fabric, spots of blood was dotted, and the dried-bloodstained fabrics were hand washed with tap water, soaked in tap water with detergent and then hand washed, for 5 and 10 min. Within moments it would become apparent if there was evidence of murder. The FBI's Behavioral Sciences Unit developed the process of profiling in the 1970s, and Ted Bundy was one of the first serial killers to be profiled. Thankfully their effort was fruitless, so they brought everything to Abby before taking a well-deserved break. DNA, however, can make or break a case. Luminol can detect the small amount of blood present in urine, and can be distorted if animals' urine is present in the room being tested. Results of ABO blood grouping from dried blood- stains treated with luminol reagent by absorption elution technique (n = 35). Yeshion, T. The reaction is used by criminalists to detect traces of blood at crime scenes. There are chemicals that react with these proteins, such as Luminol, (from the word Luminous)…The luminol "highlights" these proteins which can be seen by using a black light…even through paint. Luminol reacts with Bleach as well, but at a slower rate. When all else fails, consider getting a tracking dog (where legal) or use a luminol-based blood-detecting product like Bluestar. blood, determine whether it is human blood, and establish whether the blood comes from the victim or the criminal. Now imagine their luck! That very knife blade allegedly bore a speck of DNA so infinitesimal that only two things could be determined for sure. Since it is nearly impossible to clean up every trace of blood at a crime scene, luminol is especially effective at detecting minute traces of blood that may not be visible to the naked eye. Luminol is also used on police crime dramas (Dexter, etc. Determining the signature and the MO are both aspects of profiling. The luminol solution is sprayed where blood. Simply add 4 oz of distilled water per vial. Ji Yunshu stated to explain. “If you want to ascertain the presence of blood on a knife, or even on clothes, the best method would be a luminol test1. Detectives hoped to learn more using a chemical called luminol, which emits a distinctive glow when it comes in contact with blood. Just because he's the best blood splatter analyst in the Miami metro area, doesn't mean he can't be taught a thing or two about blood. The telltale glow was unmistakable. If there is trace amounts of blood in no amount of liquid except the luminol solution, it will grow brighter. Even though it is no longer night, it might as well be with the clouds, which have started to take on strange colors within their shadows. Luminol in Forensics Introduction In 1928 Chemist Albrecht discovered chemical that emitted blue glow In 1937, scientist Specht used chemical on bloody items In 1951 Grodsky used chemical with sodium carbonate to detect blood Improvement made in 1966 by Weber which replaced. A presumptive reagent for dilute blood detection other than luminol is fluorescein. I found this article (page 4-6) that explains the finding of blood at a crime scene, how luminol is used and how they can tell it is blood instead of. Free essays, homework help, flashcards, research papers, book reports, term papers, history, science, politics. Luminol reacts with Bleach as well, but at a slower rate. There are chemicals that react with these proteins, such as Luminol, (from the word Luminous)…The luminol "highlights" these proteins which can be seen by using a black light…even through paint. Taken with Canon 50mm lens. You can make saline (salt) or lye (NaOH) solutions. DNA-analysis of the blood traces is still possible after luminol treatment. These were found with Luminol which detects several substances including blood. This is the simplified reaction mechanism for the production of light by luminol. courtesy of Howstuffworks. It would be in a limited area that was cleaned by them with bleach and other chemicals. If and only if you get a positive reaction to both tests do you presume the sample may be blood, in which case it is sent to the lab to have the finding confirmed. ORDER: Ama zon B&N Books-A-Million IndieBound Kobo. 02) and healthy controls (46620, 26855, p= 0. Bloodstains will show up in a bright blue colour. Although there was no discernible motive, no confession, no eyewitnesses, and only circumstantial evidence, Darlie was convicted and sentenced to death. There were also lots of blood stains on the floor. This fluorescing capability makes luminol very useful when you are trying to detect areas where blood has been cleaned up in an attempt to hide a crime. The electrons in the 5-aminophthalic acid are in an unstable, excited. Yeshion 2004 The Use of Luminol as a Presumptive Blood Test for Prehistoric Archaeological Artifacts. DNA results revealed that one of the individuals who bled onto the necklace was Damien Echols, the chief suspect in the murders of the three victims. che 3238 october 13, 2014 luminol synthesis and chemiluminescence abstract in this experiment, luminol was synthesized and used to investigate its Sign in Register Hide. The luminol solution is sprayed where blood. Luminol is a powder made up of carbon, hydrogen, oxygen, and nitrogen. Useful in searching large areas for traces of blood. Blood stop powder for humans. There were also lots of blood stains on the floor. DNA was extracted through Chelex100 from these fabrics and quantified through Real Time PCR using the Quantifiler Human DNA Quantification kit. You're not getting this. Luminol reacts with Bleach as well, but at a slower rate. In fact, even if the shady criminal washed a bloodstained item of clothing 10 times, these chemicals could still reveal blood. The luminol chemiluminescence reaction is responsible for the glow of lightsticks. However, it is strongly recommended to use an airbrush for spraying luminol. Spotting a bloodstain is pretty easy at a crime scene, especially when there was no attempt to cover it up. Mike demonstrates how to use preliminary blood tests at a crime scene. Luminol can detect the small amount of blood present in urine, and can be distorted if animals' urine is present in the room being tested. The production of light from luminol is a fairly complex redox reaction. A pony displaced in space and time finds herself attending university in Michigan when a murder occurs. Cobalt oxyhydroxide nanoflakes with oxidase-mimicking activity induced chemiluminescence of luminol for glutathione detection. You should probably use some luminol to expose some hidden blood stains as well… Cuz the police won’t miss those! Then just leave carrying the corpse in a body bag like nothing ever happened. Positive blood results from porous combustible surface is more likely to be successful using Luminol or Bluestar. The detectives said the lab used Luminol, a chemical used by forensics specialists to detect trace amounts of blood during investigations, on a blanket and trunk of the Bardstown Police cruiser. On each fabric, spots of blood was dotted, and the dried-bloodstained fabrics were hand washed with tap water, soaked in tap water with detergent and then hand washed, for 5 and 10 min. He uses green check, hemisticks, luminol and bluestar to locate blood. The chemical showed blood on the coffee table, on the floor, in the laundry room and in the bathroom. - Luminol is utilized for some blood testing but they have used BLUE Star on the carpet stains. After cleaning the area with bleach, apply Luminol to the entire surface and walls. From MaestroVision. These were found with Luminol which detects several substances including blood. gl/y4V7w3 Chem­istry isn’t just in­ter­est­ing and im­pres­sive, it’s also a use­ful sci­e. There’s the beauty mark above Faith’s mouth. The luminol solution is sprayed where blood. Interview Recording Systems. now report that CRISPR, a DNA editing technology, corrected blood stem cells from patients with an immunodeficiency disorder (chronic granulomatous disease) caused by mutations in NOX2. So if I were in the need of cleaning blood to make it undetectable I would first use Hydrogen Peroxide, then Iron Out, and lastly rubbing alcohol. Suddenly you hear the killer 's (Most likely) footsteps closing in. How does Luminol indicate the presence of blood? What are the advantages and disadvantages of using Luminol? 76. Asthma severity was classified using NHLBI guidelines. This fluorescing capability makes luminol very useful when you are trying to detect areas where blood has been cleaned up in an attempt to hide a crime. Free essays, homework help, flashcards, research papers, book reports, term papers, history, science, politics. Although there was no discernible motive, no confession, no eyewitnesses, and only circumstantial evidence, Darlie was convicted and sentenced to death. "I'm gonna need some forensic experts over here, make sure they bring the luminol. The most popular current method for detecting hidden stains made by blood and other bodily fluids at crime scenes involves a substance known as luminol, which glows, or “fluoresces,” when. A total of 200 μl of human blood in a heparin tube were transferred into a tube containing 1,800 μl of blood lysis buffer (0. Substances other than blood (2–6) can catalyse the CL reaction that is the cen-tral component of the luminol test. Paint will "cover" blood, but,the blood is still there and painting over the blood actually seals the proteins in place. "There is some of Peter Falconio’s blood on the road and on small items on the side of the road. So I guess there are other substances that react with luminol and light up underneath it. too much luminol). She also shows how the chemical Luminol can reveal hidden blood stains that the eye alone cannot see. The smell of luminol hitting his him hard as the entire room had traces of fluorescent blue glow, showing up evidence of a fairly big blood spatter under the ALS light. As soon as possible, dial 911 to report the crime and to summon medical and law enforcement help. Editorial Policies; Author Guidelines. DNA, however, can make or break a case. Peng Sijue repeatedly checked and noted, "The left side of the closet door and the left side of the ground have splattered blood. If you think your deer is still alive, send a shooter around and ahead of the animal to try to dispatch it as you continue tracking. 11-02 9 samples of Luminol-revealed blood traces were taken from the cottage, including Knox’s room, the corridor and Romanelli’s room. Combining a bluesy swagger and rock n roll beat with proper honky-tonk style piano, it features Blood on lead vocals with Layne performing backing duties. Alveolar macrophages (AM) appear to be one type of cells of the bronchial inflammation often involved in patients with asthma. The electrons in the 5-aminophthalic acid are in an unstable, excited. "The blood that was on the road consisted of three pools - one larger pool and two smaller pools adjacent," he says. From a trial lawyer’s perspective, determination of whether luminol was used in a case is a critical issue for a couple of reasons. I mean, there was blood everywhere apparently, even tracked outside, not cleaned up or anything. Luminol wouldn't find it if they cleaned it decently. Criminalistics Unit 3 Jeopardy Style Review Game. They were tested with TMB which is more specific for blood. For MEL Science safe experiments subscription go here: https://goo. What about Luminol used in the search? Did they spray Luminol in the home and/or over the carpet in the SUV to detect blood? If they did, and a small amount of blood was only found in the SUV, then no murder occurred here. The Luminol Blood Test is considered a highly-sensitive widely-used screening test for blood on surfaces and is used [usually at a crime scene] to test for the presence of blood on surfaces even when blood is not visible to the naked eye under normal lighting. I know this is true. In any good-sized agency, there are specially trained and designated personnel that collect eviden. Blood with green or brown matter in it indicates a liver shot. Although it was very carefully wiped away, it was completely revealed due to the luminol. When the scene has been wiped clean, there are still ways for forensic investigators to detect washed away blood, like using a reagent called luminol, which reacts with iron found in hemoglobin. With the amount of bleeding present, this was obviously the first crime scene. 1991 Bioluminescence and Chemiluminescence: Current Status. Pig blood is very close in thickness to human blood. blood, determine whether it is human blood, and establish whether the blood comes from the victim or the criminal. How to Use Instant Jeopardy Review: Instant Jeopardy Review is designed for live play with up to ten individuals or teams. hide evidence, perpetrators can wash and destroy bloodstains,. "Oxy" cleaners may make forensic tests, such as this one checking a shoeprint for blood, ineffective. The luminol solution is sprayed where blood. In an alkaline solution luminol exists in equilibrium with its anion. They then used luminol and black light to find otherwise invisible trace material and searched the rest of the apartment thoroughly for more hidden spaces. The towels were used in cleaning up a homicide scene. Through BRET between the luminol and Ce6 units, luminescence originating from the energy donor luminol upon triggering by inflammatory mediators of ROS and MPO may excite the acceptor Ce6 to produce fluorescence and singlet oxygen (1O2). They look for standard evidence like fingerprints, footprints or blood at the scene of a […]. Home; About the Journal. Do what is necessary to keep yourself out of harm’s way. The iron in the blood serves as the catalyst for the luminol reaction. If blood is present, you get a pale blue luminescence. An archery stabilizer reduces the amount of shock and recoil caused by the relea. Luminol reacts with faecal matter, causing the same glow as if it were blood. That said, a persons blood (and it does not say if it was field tested following luminol ( and did I miss something about Jennifer Graves and moving a couch and a carpet stain not in these docs or am I blurring a case) in itself, unless their is a origin or volume amount to render the presumption of death ( meaning whatever is found indicates a. Even on luminol assay, after PMA stimulation, there was higher ROS production in naïve-RA than MTX-RA. Ema seems to have an idea she wants to try out and shows Phoenix a bottle of Luminol spray; a spray that can reveal traces of blood even after they have been washed away. Proven Ways to Remove Blood Stains. The analyst would then wait and check the sample under a microscope. The chemical luminol, a component of BLUESTAR®, is oxidized by hydrogen peroxide, to produce a molecule in an excited state. ) to detect hidden blood spatters when spayed with Luminol and exposed to UV light. Luminol Blood Search Reagent – New Prices! From Sirchie. Spotting a bloodstain is pretty easy at a crime scene, especially when there was no attempt to cover it up. If the small, characteristically shaped crystals form in the sample, it is blood. 뭐 하는지 알겠군요 피를 찾고 있는 거요? My husband didn't do anything. Offer first aid to anyone who’s injured. Luminol is oxidized after injection of 10 uM sodium hypochlorite to emit a 2-second light flash that is amplified by H2O2. When Luminol testing of the car was conducted the day after it was found, a positive reaction for blood was detected from the passenger compartment, seat, and floorboard. The production of light from luminol is a fairly complex redox reaction. Luminol is also used on police crime dramas (Dexter, etc. Experienced investigators can make a reliable identification based on how quickly the reaction occurs. You should probably use some luminol to expose some hidden blood stains as well… Cuz the police won’t miss those! Then just leave carrying the corpse in a body bag like nothing ever happened. Light Electrons are energized and photons are released to make light The electrons in higher orbitals emit a photon…. Watch trailers & learn more. which means she tried to hide and he ends up found her. There is a reason so many articles are written on the subject of cleaning blood – everyone is desperate to know the secret. Subsequent applications of Luminol will be ineffective. Many cleaning products such as those used to clean showers will cause luminol to react. First of all, luminol does not react with blood alone. All blood vials have a hole in the top- it's how the blood gets in The evidence seal on the blood vial's box was broken in a meeting with Avery's own lawyers from his wrongful conviction suit The FBI did release a r eport stating the detection levels of their EDTA test, and it was sensitive enough to find EDTA in a volume just 1% of what was in. What is the difference between polyclonal and monoclonal antibodies? How are each produced?. doc file with office 2007 say abc. ) to detect hidden blood spatters when spayed with Luminol and exposed to UV light. The Luminol is indeed reacting with the iron in the hemoglobin, and part of the mixture that makes Luminol is Hydrogen peroxide. However, it is strongly recommended to use an airbrush for spraying luminol. A presumptive reagent for dilute blood detection other than luminol is fluorescein. “Oxy” cleaners may make forensic tests, such as this one checking a shoeprint for blood, ineffective. eu jpfipes. There is a reason so many articles are written on the subject of cleaning blood - everyone is desperate to know the secret. The luminol molecule reacts with hydroxide molecules (OH-) to form the dianion (two negative charges). A good lawyer can contest circumstantial evidence and some physical evidence as well, including luminol findings. Luminol is not fairly toxic and NMRs are used in characterization, not synthesis. Luminol is used by forensic investigators to detect trace amounts of blood left at crime scenes. As soon as possible, dial 911 to report the crime and to summon medical and law enforcement help. Blood stop powder for humans. Many cleaning products such as those used to clean showers will cause luminol to react. for diluting the blood by spraying too much Luminol. To spray the Luminol, use to touch the areas you want to test. HISTORY • Luminol is a liquid solution that is sprayed to reveal traces of blood at crime scenes. What I say out loud: “Did you know that blood doesn’t naturally fluoresce? It will not show up under a blacklight like in the movies. In an attempt to hide evidence, perpetrators can wash bloodstains, making them invisible to the naked eye. Ji Yunshu stated to explain. "The blood that was on the road consisted of three pools - one larger pool and two smaller pools adjacent," he says. 9% received blood transfusions. The key to cleaning blood is a 10:1 bleach solution. 뭐 하는지 알겠군요 피를 찾고 있는 거요? My husband didn't do anything. "With a little practice, BLUESTAR® FORENSIC makes it impossible to get confused between blood and false positives since the luminescence differs in color. Students conduct a series of labs which teaches them about evidence gathering and determing on-scene if a sample is potentially blood. The dianion, which exists in two forms (the two-way arrow), reacts with oxygen to form 5-aminophthalic acid and nitrogen gas (N 2). DNA, however, can make or break a case. 11-01 1 sample of a Luminol-revealed blood trace was taken from Guede’s apartment. courtesy of Howstuffworks. David says:. Unfortunately, most of the articles written. You know what I mean, it's in those light sticks you can snap to get colored light for a couple of hours. 6) Luminol Powder Vials (reconstitutes to a total of 24 oz) Chemical Shelf Life. How do precipitin and gel diffusion tests indicate whether blood is from a human or a specific type of animal? 77. They look for standard evidence like fingerprints, footprints or blood at the scene of a […]. gl/y4V7w3 Chem­istry isn't just in­ter­est­ing and im­pres­sive, it's also a use­ful sci­e. The production of light from luminol is a fairly complex redox reaction. A boning knife that was inserted on the knife rack. A gallium spoon melts in hot water. Cobalt oxyhydroxide nanoflakes with oxidase-mimicking activity induced chemiluminescence of luminol for glutathione detection. Don't even think of writing a medical mystery without the wise counsel of Dr. " Four hundred and sixty-three of these criminals have been found. Blood stop powder for humans. A pony displaced in space and time finds herself attending university in Michigan when a murder occurs. Although it can detect small traces of blood, forensic investigators use this only as a last resort. Every order is not just a body to ditch, but also a race against time. From The Deep Sea to the Crime Lab Luminescence 2. The luminol chemiluminescence reaction is responsible for the glow of lightsticks. Thus, by reporting positive luminol results, suggesting but not conclusively demonstrating the presence of blood, but withholding the negative TMB results, which demonstrated the absence of blood and are therefore exculpatory, the forensic police and prosecution misrepresented the evidence for nearly the entire length of the first-level trial. Watch trailers & learn more. I know this is true. The Luminol is indeed reacting with the iron in the hemoglobin, and part of the mixture that makes Luminol is Hydrogen peroxide. Means either only 1drop was there or report's wrong. Since its beginnings sixty years ago, four hundred ninety-four criminals have been placed on the "Top Ten List. See full list on science. Teams of investigators arrive at a crime scene armed with special fact-finding gear. Average pretransfusion hametocrit (Hct) was 24. In newly released evidence, a photo entitled "luminol photos" show luminol, which investigators used to find cleaned up blood splattered throughout the salon. - 내 마룻바닥이 젖잖아요 - 그건 루미놀이랍니다. For Luminol: It's sprayed at a scene (in the dark) HOW TO DETECT BLOOD OR BODILY FLUIDS USING UV LIGHT. Choice of Catalyst; 20/A, 45, 77, 9, B13/28, B3. Luminol reacts to fecal matter as well as blood, so it's no help. You attract them just like you attract any other blood-sucking insects. Results of ABO blood grouping from dried blood- stains treated with luminol reagent by absorption elution technique (n = 35). 1991 Bioluminescence and Chemiluminescence: Current Status. These were found with Luminol which detects several substances including blood. Transfused patients were more likely to have been taken back to the operating room. Yeshion 2004 The Use of Luminol as a Presumptive Blood Test for Prehistoric Archaeological Artifacts. How to Use Instant Jeopardy Review: Instant Jeopardy Review is designed for live play with up to ten individuals or teams. PaO2 < 100 (FiO2 >= 21%) PaCO2 of 30-40. My husband is a maintenance man for a hotel and has been given the task of removing all luminol used in an investigation there. Talk with your doctor. So far, no luck. It is used at crime scenes and makes a major mess. Luminol is not fairly toxic and NMRs are used in characterization, not synthesis. Chlorine-based detergents contaminate blood, but leave behind intact hemoglobin. If I disappeared and forensic investigators used Luminol on my shower, would they think I was murdered in there? I once saw an episode of Forensic Files where the narrator said in passing that blood had been discovered in a missing woman's bathroom, "and it was not menstrual. 1 fmol, respectively. “This is artery spray. There was a slight discolouration in the white paint, only a minuscule difference, barely noticeable; but the man's trained eyes didn't miss anything. • Several chemicals in luminol react with the hemoglobin found on the crime scene, resulting in a light-producing chemical reaction. It reacts to the Luminol, showing another handprint. A personal note: “I never visit chic stores. Luminol reacts with oxygen. De Ravin et al. The luminol set F-31000 is based on a Dutch recipe. Luminol must be used very cautiously because once it touched blood and chemically reacted, at the same time it would also affect the follow-up testing of the blood contents. 11-02 9 samples of Luminol-revealed blood traces were taken from the cottage, including Knox’s room, the corridor and Romanelli’s room. Yeshion, T. Simply add 4 oz of distilled water per vial. DNA-analysis of the blood traces is still possible after luminol treatment. "There is some of Peter Falconio’s blood on the road and on small items on the side of the road. Nonetheless, unlike in CSI, luminol alone will not solve a crime. A presumptive reagent for dilute blood detection other than luminol is fluorescein. (B) Sketch of self-assembly of the CLP conjugate into a core-shell structured nanoparticle. Luminol testing also reacts to coppery substances, plant enzymes and excrement, raising an alternate explanation for the luminol results in the hallway. "I'm gonna need some forensic experts over here, make sure they bring the luminol. But it is safer for the scientists to use. BLOOD JEOPARDY Jeopardy Style Review Game. My husband is a maintenance man for a hotel and has been given the task of removing all luminol used in an investigation there. Students conduct a series of labs which teaches them about evidence gathering and determing on-scene if a sample is potentially blood. Growth of CD34+ PBPs for 28 days in the. The Blood Lamp arouses fear with just the mention of its name; the unique lamp demands the blood of the user as payment before it will cast its bluish glow on you. Pig blood is very close in thickness to human blood. Judge Nencini pointed out that it’s utterly inconceivable that the Luminol revealed anything other than blood, given the undisputed presence of a lot of blood at the cottage. One of these tests consists of spraying a suspected sample with a solution of luminol (c8H7N3O2), a chemical popularized by the TV series "csI" (short for "crime scene Inves-tigation"), and hydrogen peroxide (H2O2). A pony displaced in space and time finds herself attending university in Michigan when a murder occurs. We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. Designed to provide all essential materials required for the chemiluminescent Luminol method. ” “What is that… lum. On each fabric, spots of blood was dotted, and the dried-bloodstained fabrics were hand washed with tap water, soaked in tap water with detergent and then hand washed, for 5 and 10 min. peroxidase activity. It is used by forensic scientists to detect blood at crime scenes. 1% TritonX-100 in DDW) and mixed well. How do precipitin and gel diffusion tests indicate whether blood is from a human or a specific type of animal? 77. Just because he's the best blood splatter analyst in the Miami metro area, doesn't mean he can't be taught a thing or two about blood. Luminol is not fairly toxic and NMRs are used in characterization, not synthesis. Ji Yunshu stated to explain. On the sink The sink with one Knox and two mixed traces. Carry some hydrogen peroxide in a small spray bottle and it might help you pick up the trail. Report comment. Judge Nencini pointed out that it’s utterly inconceivable that the Luminol revealed anything other than blood, given the undisputed presence of a lot of blood at the cottage. The blood was too small at that time for exact DNA testing to be performed, but once again found a DNA mixture composited from blood from two different people on this necklace. Editorial Policies; Author Guidelines. If there are trace amounts of blood in a large amount of liquid with some luminol, the solution will glow faintly. They look for standard evidence like fingerprints, footprints or blood at the scene of a […]. A crime scene photograph can tell a lot of stories. PRAISE FOR FORENSICS AND FICTION. Enzymes in a variety of biological systems may also catalyse the decomposition of hydrogen peroxide. Determining the signature and the MO are both aspects of profiling. We optimized the simple human blood lysis procedure for the LAMP reaction to simplify the DNA/RNA preparation process. eu jpfipes. BLOOD JEOPARDY Jeopardy Style Review Game. Five traces of Knox DNA mixed with the victim's were found at the crime scene: three in the bathroom (along with a Knox-only bloodstain on the faucet Knox claimed not to recognize), a mixed sample in a Luminol trace in the hall, and in a Luminol trace in Romanelli's room. ORDER: Ama zon B&N Books-A-Million IndieBound Kobo. First of all, luminol does not react with blood alone. Interview Recording Systems. However, without this mean, using a solution which combines vinegar and alcohol would give a similar effect. Through BRET between the luminol and Ce6 units, luminescence originating from the energy donor luminol upon triggering by inflammatory mediators of ROS and MPO may excite the acceptor Ce6 to produce fluorescence and singlet oxygen (1O2). How to Use Instant Jeopardy Review: Instant Jeopardy Review is designed for live play with up to ten individuals or teams. That said, a persons blood (and it does not say if it was field tested following luminol ( and did I miss something about Jennifer Graves and moving a couch and a carpet stain not in these docs or am I blurring a case) in itself, unless their is a origin or volume amount to render the presumption of death ( meaning whatever is found indicates a. The food fight would have had to include horseradish, that weird spicy relative of mustard that gets smeared on roast beef, but it also glows under luminol, just like blood does. A boning knife that was inserted on the knife rack. Bloodstains will show up in a bright blue colour. The middle shoe was processed with luminol after it had been cleaned. too much luminol). To spray the Luminol, use to touch the areas you want to test. But it is safer for the scientists to use. Might not even have been blood made those prints, tho I can't think of what else a foot would step in to leave luminol traces. Luminol is also used on police crime dramas (Dexter, etc. Crime Scene Chemistry – Luminol, Blood & Horseradish. Since it is nearly impossible to clean up every trace of blood at a crime scene, luminol is especially effective at detecting minute traces of blood that may not be visible to the naked eye. A total of 200 μl of human blood in a heparin tube were transferred into a tube containing 1,800 μl of blood lysis buffer (0. Bloodstains will show up in a bright blue colour. 50-120R Enthone Quart White. Several chemicals in luminol react with the hemoglobin found on the crime scene, resulting in a light-producing chemical reaction. What about Luminol used in the search? Did they spray Luminol in the home and/or over the carpet in the SUV to detect blood? If they did, and a small amount of blood was only found in the SUV, then no murder occurred here. So, luminol glows more or less depending on the concentration of the blood in the solution. Useful in searching large areas for traces of blood. David says:. The stains detected with luminol can be attributed to no one. It is more concentrated than the classic Weber recipe and gives a stronger luminescence. {"markup":"\u003C?xml version=\u00221. Designed to provide all essential materials required for the chemiluminescent Luminol method. "I'm gonna need some forensic experts over here, make sure they bring the luminol. Blood wouldn't be everywhere. The luminol chemiluminescence reaction is responsible for the glow of lightsticks. Taken with Canon 50mm lens. Antiquity 66:24-35. Use club soda, hydrogen peroxide, ammonia, or baking soda. [Editor’s note: Luminol can also be triggered by copper, excessive cigarette smoke, horseradish sauce, fecal matter, and certain bleaches. Therefore, if blood is present, an intense light-blue glow will appear. If the crystals do not form then the test has failed to indicate blood. The most popular current method for detecting hidden stains made by blood and other bodily fluids at crime scenes involves a substance known as luminol, which glows, or “fluoresces,” when. Free essays, homework help, flashcards, research papers, book reports, term papers, history, science, politics. Bloody rags and tissues were found in the trash nearby, and a bloody women's shirt was in the laundry hamper. A crime scene photograph can tell a lot of stories. The sensitivity of fluorescein approaches the sensitivity of detection levels of luminol. A total of 200 μl of human blood in a heparin tube were transferred into a tube containing 1,800 μl of blood lysis buffer (0. 빨리 마릅니다 Surface is non-reactive. If luminol reveals apparent blood traces, investigators will photograph or videotape the crime scene to record the pattern. howstuffworks. For example, hydrogen peroxide reacts when it contacts blood, causing a foaming effect. The amount of catalyst necessary for the reaction to occur is very small, relative to the amount of luminol, allowing the detection of even trace amounts of blood. Luminol testing also reacts to coppery substances, plant enzymes and excrement, raising an alternate explanation for the luminol results in the hallway. These were undated stains that were most likely made at a different time from someone walking around after taking a shower. It was clear that there was blood everywhere, even on the glass frame of Kelly’s masters degree on the wall. She sprays a spot on the ladder a few times as an example, and then passes the buck to you. The back of the SUV had been vacuumed. A gallium spoon melts in hot water. There's not going to be blood splatter all over the equipment or ceiling. Luminol is only considered as a presumptive test for blood because there are other substances capable of giving a positive reaction with luminol itself; these other substances may be bleach, plant peroxides and some metals, the reaction between luminol and these other substances are called false positives [7]. If you don't do the Luminol, I would definitely try something like Noopept, but it is strong, and you may need something like the Picamilon to balance that out, or Phenibut HCL, which is just GABA, but with the phenyl attachment so that it crosses the blood brain barrier right away, to add a natural calm that works right away. It is used at crime scenes and makes a major mess. With each spray of the Luminol, the detectives followed a blood path of a body being dragged through the hallway and out through the children’s bedroom window. Substances other than blood (2-6) can catalyse the CL reaction that is the cen-tral component of the luminol test. Legal Brief: Improving luminol blood detection in forensics. Avoid drinking alcohol while taking Luminal (phenobarbital injection). Every order is not just a body to ditch, but also a race against time. They were tested with TMB which is more specific for blood. Steve Krejci. Carry some hydrogen peroxide in a small spray bottle and it might help you pick up the trail. In this area, there was a knife. hide evidence, perpetrators can wash and destroy bloodstains,. Determining the signature and the MO are both aspects of profiling. Ever wonder how detectives find people's fingerprints at a crime scene? It's actually not as hard as you may think. In this test, luminol powder (C 8 H 7 O 3 N 3) is mixed with hydrogen peroxide (H 2 O 2) and a hydroxide (e. If the crystals do not form then the test has failed to indicate blood. 11-02 9 samples of Luminol-revealed blood traces were taken from the cottage, including Knox’s room, the corridor and Romanelli’s room. Darlie Routier is an innocent mother who survived a brutal attack that left her two young children dead. To detect invisible blood stains, the luminol test is used, which is a chemical sprayed on carpets and furniture which reveals a slight phosphorescent light in the dark where bloodstains (and certain other stains) are present. If the small, characteristically shaped crystals form in the sample, it is blood. PRAISE FOR FORENSICS AND FICTION. To spray the Luminol, use to touch the areas you want to test. 50-120R Enthone Quart White. Ji Yunshu stated to explain. 6) Luminol Powder Vials (reconstitutes to a total of 24 oz) Chemical Shelf Life. It is more concentrated than the classic Weber recipe and gives a stronger luminescence. Luminol reacts with Bleach as well, but at a slower rate. Useful in searching large areas for traces of blood. The FBI's Behavioral Sciences Unit developed the process of profiling in the 1970s, and Ted Bundy was one of the first serial killers to be profiled. So I guess there are other substances that react with luminol and light up underneath it. Free essays, homework help, flashcards, research papers, book reports, term papers, history, science, politics. There’s the beauty mark above Faith’s mouth. How to Use Instant Jeopardy Review: Instant Jeopardy Review is designed for live play with up to ten individuals or teams. 9% received blood transfusions. Designed to provide all essential materials required for the chemiluminescent Luminol method. Since I am a woman, I get blood on my shower floor every month. Get to the stain while the blood is fresh. There was a slight discolouration in the white paint, only a minuscule difference, barely noticeable; but the man's trained eyes didn't miss anything. Luminol reacts with oxygen. DNA, however, can make or break a case. Subsequent applications of Luminol will be ineffective. These were found with Luminol which detects several substances including blood. DNA results revealed that one of the individuals who bled onto the necklace was Damien Echols, the chief suspect in the murders of the three victims. "Well, there goes another anomaly," Agent Luminol remarked to himself as he took out the "Neutralized" stamp and stamped it on the Object Class of the SCP report in front of him. 0\u0022 encoding=\u0022UTF-8\u0022 ?\u003E \u003Chtml version=\u0022HTML+RDFa+MathML 1. "Luminol proved blood was rinsed down the sink and wiped up from the counter on the sides of the sink, the taps and spout. The respiratory burst of bronchoalveolar lavage AM was studied in 12 subjects with asthma and in five control. Detectives hoped to learn more using a chemical called luminol, which emits a distinctive glow when it comes in contact with blood. Ema seems to have an idea she wants to try out and shows Phoenix a bottle of Luminol spray; a spray that can reveal traces of blood even after they have been washed away. ORDER: Ama zon B&N Books-A-Million IndieBound Kobo. Get to the stain while the blood is fresh. "The blood that was on the road consisted of three pools – one larger pool and two smaller pools adjacent," he says. Detectives hoped to learn more using a chemical called luminol, which emits a distinctive glow when it comes in contact with blood. Real forensic investigators detect bloodstains by spraying chemicals like Luminol, which react with the iron in hemoglobin and cause the bloodstains to glow. The stains mentioned by Harry all tested negative for blood. Blood stop powder for humans. PaO2 < 100 (FiO2 >= 21%) PaCO2 of 30-40. On each fabric, spots of blood was dotted, and the dried-bloodstained fabrics were hand washed with tap water, soaked in tap water with detergent and then hand washed, for 5 and 10 min. Choice of Catalyst; 20/A, 45, 77, 9, B13/28, B3. Use club soda, hydrogen peroxide, ammonia, or baking soda. The oxygen produced from the hydrogen peroxide then reacts with the luminol dianion. One of these tests consists of spraying a suspected sample with a solution of luminol (c8H7N3O2), a chemical popularized by the TV series “csI” (short for “crime scene Inves-tigation”), and hydrogen peroxide (H2O2). When chemically treated, blood can also fluoresce, such as with luminol, a reagent that reacts with heme in the hemoglobin of blood. Blood that sits around on the floor for a few minutes will form clots like big sheets of instant pudding. When the scene has been wiped clean, there are still ways for forensic investigators to detect washed away blood, like using a reagent called luminol, which reacts with iron found in hemoglobin. "He is trying to find a place to hide her body … he is trying to find a means of disposing of her body. Maybe it was Theresa who did it before she vanished. Asthma severity was classified using NHLBI guidelines. “If you want to ascertain the presence of blood on a knife, or even on clothes, the best method would be a luminol test1. (B) Sketch of self-assembly of the CLP conjugate into a core-shell structured nanoparticle. DNA, blood, luminol and subsequent circumstantial evidence after the murder of 2 Nov 2007 meant Knox was charged, together with Sollecito a few months later in the following year.